Workshop 5: Epithelial Cell Culture

Day 1:

Lectures:

1. Cultural Characteristics of different cell populations
2. Basics of Epithelial cell culture

Practical:

1. Isolation of epithelial cells from tissue biopsy
2. Initiation of culture

Day 2:

Lectures:

1. Identification of epithelial cells in culture
2. Propagation of epithelial cell cultures

Practical:

1. Cell passaging
2. Cytochemical staining