Workshop 1:  Differentiation of MSCs

Day 1:

Lectures: 

1. Origin and Isolation of MSCs
2. Characteristics of MSCs

Practical Session:            

1. Verification of MSCs before differentiation:
2. Morphology
3. Count and Viability

Day 2:

Lectures:

1. Stem Cell Transdifferentiation:  theories and practice
2. Trilineage differentiation of MSCs

Practical Sessions:

1. Osteogenic differentiation
2. Chondrogenic differentiation
3. Adipognic Differentiation

Day 3:

Lectures:

1. Evaluation of Stem Cell Plasticity
2. Evaluation of MSC trilineage differentiation

Practical:

1. Immunohistochemistry for osteogenic differentiation
2. Immunohistochemistry for chrondrogenic differentiation
3. Immunohistochemistry for adipogenic differentiation