Workshop 4:  Standardization of HSCs

Day 1:

Lectures:

1. Types and Sources of HSCs
2. Identification of HSCs
3. Methods of viability detection

Practical:

1. Mononuclear Cell Separation
2. MSCs isolation
3. Stem Cell count and viablity

Day 2:

Lectures:

1. Significance of Flowcytometry in stem cell research
2. Flowcytometry:  basic principles

Practical:

1. Gating for stem cell experiments
2. Surface Monoclonal antibody detection using   flowcytometry

Day 3:

Lectures:

1. Surface and Intracellular antigen detection using       flowcytometry
2. Cell sorting theories and methods

Practical:

1. Immunomagnetic cell sorting
2. Stem Cell count using flowcytometry (ISCHage protocol)