{"id":472,"date":"2017-12-07T10:54:19","date_gmt":"2017-12-07T10:54:19","guid":{"rendered":"http:\/\/espcr.com\/?p=472"},"modified":"2018-02-19T11:03:16","modified_gmt":"2018-02-19T11:03:16","slug":"workshop-4-standardization-of-hscs","status":"publish","type":"post","link":"https:\/\/espcr.com\/?p=472","title":{"rendered":"Workshop 4:\u00a0 Standardization of HSCs"},"content":{"rendered":"<p><strong><em>Day 1:<\/em><\/strong><\/p>\n<p><strong>Lectures:<\/strong><\/p>\n<ol>\n<li><strong> Types and Sources of HSCs<\/strong><\/li>\n<li><strong> Identification of HSCs<\/strong><\/li>\n<li><strong> Methods of viability detection<\/strong><\/li>\n<\/ol>\n<p><strong>Practical:<\/strong><\/p>\n<ol>\n<li><strong> Mononuclear Cell Separation<\/strong><\/li>\n<li><strong> MSCs isolation<\/strong><\/li>\n<li><strong> Stem Cell count and viablity<\/strong><\/li>\n<\/ol>\n<p><strong><em>Day 2:<\/em><\/strong><\/p>\n<p><strong>Lectures:<\/strong><\/p>\n<ol>\n<li><strong> Significance of Flowcytometry in stem cell research<\/strong><\/li>\n<li><strong> Flowcytometry:\u00a0 basic principles<\/strong><\/li>\n<\/ol>\n<p><strong>Practical:<\/strong><\/p>\n<ol>\n<li><strong> Gating for stem cell experiments<\/strong><\/li>\n<li><strong> Surface Monoclonal antibody detection using \u00a0 flowcytometry<\/strong><\/li>\n<\/ol>\n<p><strong><em>Day 3:<\/em><\/strong><\/p>\n<p><strong>Lectures:<\/strong><\/p>\n<ol>\n<li><strong> Surface and Intracellular antigen detection using \u00a0\u00a0\u00a0\u00a0\u00a0 flowcytometry<\/strong><\/li>\n<li><strong> Cell sorting theories and methods<\/strong><\/li>\n<\/ol>\n<p><strong>Practical:<\/strong><\/p>\n<ol>\n<li><strong> Immunomagnetic cell sorting<\/strong><\/li>\n<li><strong> Stem Cell count using flowcytometry (ISCHage protocol)<\/strong><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Day 1: Lectures: Types and Sources of HSCs Identification of HSCs Methods of viability detection Practical: Mononuclear Cell Separation MSCs isolation Stem Cell count and viablity Day 2: Lectures: Significance of Flowcytometry in stem cell research Flowcytometry:\u00a0 basic principles Practical: Gating for stem cell experiments Surface Monoclonal antibody detection using \u00a0 flowcytometry Day 3: Lectures: &hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[9],"tags":[],"class_list":["post-472","post","type-post","status-publish","format-standard","hentry","category-workshops"],"_links":{"self":[{"href":"https:\/\/espcr.com\/index.php?rest_route=\/wp\/v2\/posts\/472","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/espcr.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/espcr.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/espcr.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/espcr.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=472"}],"version-history":[{"count":1,"href":"https:\/\/espcr.com\/index.php?rest_route=\/wp\/v2\/posts\/472\/revisions"}],"predecessor-version":[{"id":473,"href":"https:\/\/espcr.com\/index.php?rest_route=\/wp\/v2\/posts\/472\/revisions\/473"}],"wp:attachment":[{"href":"https:\/\/espcr.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=472"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/espcr.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=472"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/espcr.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=472"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}