{"id":314,"date":"2017-12-09T14:29:35","date_gmt":"2017-12-09T14:29:35","guid":{"rendered":"http:\/\/espcr.com\/?p=314"},"modified":"2018-02-19T11:05:30","modified_gmt":"2018-02-19T11:05:30","slug":"workshop-2","status":"publish","type":"post","link":"https:\/\/espcr.com\/?p=314","title":{"rendered":"Workshop 2.\u00a0 IPS"},"content":{"rendered":"

Day 1:<\/em><\/strong><\/p>\n

Lectures:<\/strong><\/p>\n

    \n
  1. Basic Stem Cell Biology<\/strong><\/li>\n
  2. Pleuripotency: pathways and detection<\/strong><\/li>\n<\/ol>\n

    Practical:<\/strong><\/p>\n

    \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0 Fibroblast culture:<\/strong><\/p>\n

      \n
    1. Isolation of fibroblasts from skin samples<\/strong><\/li>\n
    2. Culture and propagation of fibroblasts<\/strong><\/li>\n
    3. Characterization of fibroblasts in culture<\/strong><\/li>\n<\/ol>\n

      Day 2:\u00a0 <\/em><\/strong><\/p>\n

      Lectures:<\/strong><\/p>\n

        \n
      1. IPS:\u00a0 theory and applications<\/strong><\/li>\n
      2. Methods of induction of IPS<\/strong><\/li>\n<\/ol>\n

        Practical:<\/strong><\/p>\n

          \n
        1. Transfection of cells for pleuripotency<\/strong><\/li>\n
        2. Culture of transfected cells<\/strong><\/li>\n<\/ol>\n

          Day 3:<\/strong><\/p>\n

          Lectures:<\/strong><\/p>\n

            \n
          1. Methods of selection of transfected cells<\/strong><\/li>\n
          2. Propagation of pleuripotent cells<\/strong><\/li>\n<\/ol>\n

            Practical:<\/strong><\/p>\n

              \n
            1. Kill curve plotting<\/strong><\/li>\n
            2. Culture of viable cells<\/strong><\/li>\n
            3. RNA extraction from selected cells<\/strong><\/li>\n<\/ol>\n


              \n<\/u><\/em><\/strong><\/p>\n

              \u00a0<\/u><\/em><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

              Day 1: Lectures: Basic Stem Cell Biology Pleuripotency: pathways and detection Practical: \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0 Fibroblast culture: Isolation of fibroblasts from skin samples Culture and propagation of fibroblasts Characterization of fibroblasts in culture Day 2:\u00a0 Lectures: IPS:\u00a0 theory and applications Methods of induction of IPS Practical: Transfection of cells for pleuripotency Culture of transfected cells Day …<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[9],"tags":[],"class_list":["post-314","post","type-post","status-publish","format-standard","hentry","category-workshops"],"_links":{"self":[{"href":"https:\/\/espcr.com\/index.php?rest_route=\/wp\/v2\/posts\/314","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/espcr.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/espcr.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/espcr.com\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/espcr.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=314"}],"version-history":[{"count":3,"href":"https:\/\/espcr.com\/index.php?rest_route=\/wp\/v2\/posts\/314\/revisions"}],"predecessor-version":[{"id":470,"href":"https:\/\/espcr.com\/index.php?rest_route=\/wp\/v2\/posts\/314\/revisions\/470"}],"wp:attachment":[{"href":"https:\/\/espcr.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=314"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/espcr.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=314"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/espcr.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=314"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}